BIOMASS TO ETHANOL
BIOMASS TO ETHANOL: HYDROLYSIS AND
FERMENTATION
Sugars are the essential raw material for a range of
biobased products from ethanol to bioplastics. In the
case of ethanol, sugar is converted into alcohol through
fermentation. Both corn grain and cellulosic feedstocks,
such as corn stover, straw and wood, are composed of
about 70 percent sugars, making them good candidates
for ethanol production. The challenge lies in extracting
the sugars from these agricultural feedstocks.
In corn grain, the sugars are all of the same variety (6-
carbon molecules of glucose), joined together with
relatively simple bonds to form starch. These simple
bonds can easily be broken using commonly available
amylase enzymes and water in a process called hydrolysis.
Isolating the sugars in cellulosic biomass is a
considerably more complicated task. Cellulosic biomass is
composed of a mixture of 6-carbon glucose sugars in the
form of cellulose and 5-carbon pentose sugars linked to
other 6-carbon sugars in the form of hemicellulose, all
held together by complex chemical bonds bound with a
stiff, fibrous substance called lignin. The biomass must
first be pre-treated to separate the lignin and loosen the
chemical bonds. Cellulase enzymes can then be used to
break the sugar-to-sugar bonds via hydrolysis.
Recent biotech advances have made significant
improvements in cellulase enzymes and pentoseprocessing
microbes, closing the gap on making cellulosic
biomass conversion to ethanol economical. As
commercialization proceeds, further gains will help ensure
sustainable feedstock platforms for fuels and chemicals.
Sugar
content
70% as starch. 30-50% as cellulose.
25-32% as hemicellulose.
Conversion
to individual
sugars
Straightforward conversion
to sugars via amylase
enzymes.
Current starch to sugar
conversion cost 3¢ to
5¢ per gallon ethanol.
Challenge to convert to sugars.
Cellulose to glucose with muchimproved
enzymes approaching
10¢ per gallon.
Current
ethanol
yield
105 to 120 gallons
per dry ton (2.5 to 2.8
gallons ethanol per
bushel).
80 to 90 gallons per dry ton feedstock.
Pentose fermentation to alcohol
still an evolving technology.
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